Hemolysin coregulated protein 1 as a molecular gluing unit for the assembly of nanoparticle hybrid structures

• Tuan Anh Pham,
• Andreas Schreiber,
• Elena V. Sturm (née Rosseeva),
• Stefan Schiller and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2016, 7, 351–363, doi:10.3762/bjnano.7.32

• inorganic material. Here, we use hemolysin coregulated protein 1 (Hcp1) from Pseudomonas aeruginosa as a building and gluing unit for the formation of biohybrid structures by implementing cysteine anchoring points at defined positions on the protein rim (Hcp1_cys3). We successfully apply the Hcp1_cys3

• to a higher squareness value of the hysteresis curve. Thus the Hcp1_cys3 unit is shown to be very versatile in the formation of new biohybrid materials with enhanced magnetic, catalytic and optical properties. Keywords: gold catalyst; hemolysin coregulated protein 1 (Hcp1); magnetic hybrid materials

• potential in the design of 1D NP hybrid structures with advanced properties. However, examples for NP assembly using proteins are still limited [15][16][17][18]. Here, we apply the hemolysin coregulated protein 1 (Hcp1) homohexameric protein from Pseudomonas aeruginosa with its toroidal structure as a